卢丽丽
E-mail：lililu@hust.edu.cn
药学院3号楼210-212
教育及工作经历
2019.11至今，华中科技大学药学院，教授，博士生导师
2016.12至2019.10，华中科技大学药学院，副教授，博士生导师
2013.7~2014.10，美国韦恩州立大学化学系，访问学者
2010.12~2016.11，山东大学国家糖工程技术研究中心，副教授，硕士生导师
2007.10~2009.12，山东大学药学院，药学博士后
2007.7~2010.11，山东大学国家糖工程技术研究中心，讲师
2002.7~2007.6，山东大学微生物技术国家重点实验室，微生物学博士
1998.9~2002.6，湖北师范大学，生物科学学士
研究方向
生物技术制药及糖生物化学：重点以生物酶或构建含糖链加工相关生物酶的细胞工厂，进行食品、药物及疾病相关的糖链合成研究，包括功能性低聚糖、寡糖抗感染药物、肿瘤糖链相关抗原、GPI锚定蛋白等糖缀合物；此外还涉及天然产物及药物分子的糖基化修饰及药理活性研究等。
目前主持及参与国家、省部级项目近20项，参编国际专著1部，在Biotechnol Adv,Angew Chem Int Ed，Bioresource Technol，Appl Environ Microbiol，Food Chem等国内外学术刊物上发表科研论文50余篇，其中第一作者或通讯作者论文30余篇，申请(含参与)并获得国家发明专利30余项。
近三年教学工作
讲授课程：本科生及留学生《药理学》、《生物技术制药》、《生物化学》；研究生《应用药理学》。
华中科技大学教改项目：2020-2022《药理学》“线上”+“线下”的混合式教学模式探究，主持。
教学竞赛获奖：“华中科技大学2017～2018学年教师教学竞赛理论课竞赛”一等奖。
近五年科研项目
(1)2021-2024国家自然科学基金“以单糖为糖基供体的β-半乳糖苷酶资源挖掘、分子改造及糖苷合成研究”(32071473) 主持直接经费58万
(2)2020-2021糖化学与生物技术教育部重点实验室2020年度开放课题“脂磷壁酸作为疫苗载体和自身佐剂的研究” (KLCCB-KF202006) 主持2万
(3)2019-2022国家自然科学基金“GPI转酰胺酶合成GPI锚定蛋白的研究”(21877044) 主持直接经费62万
(4)2018-2022企业横向“烷基糖苷的合成工艺研究”主持60万
(5)2018-2020华中科技大学自主创新项目“酶法高效合成唾液酸相关的人乳寡糖及肿瘤糖链抗原”(2018KFYYXJJ020)主持8万
(6)2017-2019华中科技大学人才引起基金主持50万
(7)2015-2017山东省重点研发计划项目“肠出血性大肠杆菌抗感染寡糖药物的糖苷酶法合成研究”(2015GSF121004)主持15万
(8)2012-2015国家863计划项目“糖工程关键技术与重大产品开发”(2012AA021500)课题1“糖链加工与功能寡糖的研制”子课题“牛蒡低聚果糖规模化生产的关键技术研究及半乳糖苷酶的研制”主持9万
(9)2012-2016国家重点基础研究发展计划(973计划)“肿瘤的糖化学生物学前沿研究”课题2糖链探针的生物合成研究(2012CB822102) 600万参与
学术专著
Lili Lu, Min Xiao. Chapter 5 Recent Progress on Galacto-Oligosaccharides Synthesis by Microbial β-Galactosidase inFunctional Carbohydrates.CRC Press, Inc.: Boca Raton, Florida, US, 2017. 147-182
代表性论文(*corresponding author,#first author)
(1)Lili Lu*, Longcheng Guo, Ke wang, Yan Liu, Min Xiao. β-Galactosidases: A great tool for synthesizing galactose-containing carbohydrates.Biotechnol Adv.2020, 39, 107465.
(2)Yali Tuo, Zijun Zhang, Cheng Tian, Qinyu Hu, Rui Xie, Jian Yang, Hong Zhou,Lili Lu*, Ming Xiang*. Anti-inflammatory and metabolic reprogramming effects of MENK produce antitumor response in CT26 tumor-bearing mice.J Leukoc Biol.2020, 108(1):215-228.
(3)Ke wang, Tingting Qi, Longcheng Guo, Zhongxuan Ma, Guofeng, Gu, Min Xiao*,Lili Lu*. Enzymatic glucosylation of salidroside from starch by α-amylase.J Agric Food Chem. 2019, 67(7):2012-2019.
(4)Longcheng Guo, Zhongxuan Ma,Lili Lu*, Min Xiao*. Progress in catalytic mechanism of sialidases and their functions in oligosaccharide hydrolysis and synthesis.Prog Biochem Biophys.2019,46(2):181~192.
(5)Longcheng Guo, Xiaodi Chen, Li Xu, Min Xiao*,Lili Lu*. Enzymatic synthesis of 6'-Sialyllactose, a dominant sialylated human milk oligosaccharide, by a novel exo-α-sialidase fromBacteroides fragilisNCTC9343.Appl Environ Microbiol.2018, 84(13): pii: e00071-18.
(6)Tingting Qi, Guofeng Gu, Li Xu, Min Xiao*,Lili Lu*.Efficient synthesis of tyrosol galactosides by the β-galactosidase fromEnterobacter cloacaeB5.Appl Microbiol Biotechnol.2017, 101(12):4995-5003.
(7)Xiaodi Chen, Li Xu, Lan Jin, Bin Sun, Guofeng Gu,Lili Lu*, Min Xiao*. Efficient and regioselective synthesis ofβ-GalNAc/GlcNAc-lactose by a bifunctional transglycosylatingβ-N-acetylhexosaminidase fromBifidobacterium bifidum.Appl Environ Microbiol. 2016, 82(18):5642-5652.
(8)Wei Gong,Li Xu,Guofeng Gu,Lili Lu*,Min Xiao*. Efficient and regioselective synthesis of globotriose by a novel α-galactosidase fromBacteroides fragilis.Appl Microbiol Biotechnol.2016, 100(15):6693-6702.
(9)Lijuan Xu, Tingting Qi, Li Xu,Lili Lu*, Min Xiao*.Recent progress in the enzymatic glycosylation of phenolic compounds.J Carbohyd Chem.2016, 35(1):1-23.
(10)Jie Zhang#,Lili Lu#, Li Lu, Yang Zhao, Liping Kang, Xu Pang, Jingyuan Liu, Tengchuan Jiang, Min Xiao*, Baiping Ma*.Galactosylation of steroidal saponins by β-galactosidase from Lactobacillus bulgaricusL3.Glycoconj J.2016, 33(1):53-62.
(11)Lili Lu, Yuchuan Guo, Lijuan Xu, Tingting Qi, Lan Jin, Li Xu, Min Xiao. Galactosylation of caffeic acid by an engineered β-galactosidase.Drug Discoveries & Therapeutics. 2015, 9(2):123-128.
(12)Lili Lu#, Jian Gao#, Zhongwu Guo*. Labeling cell surface GPIs and GPI-anchored proteins through metabolic engineering with artificial inositol derivatives.Angew Chem Int Ed. 2015, 54(33): 9679-9682.
(13)Lili Lu#, Qian Liu#, Lan Jan, Zhenghao Yin, Li Xu, Min Xiao*.Enzymatic synthesis of rhamnose containing chemicals by reverse hydrolysis.PLoS One. 2015, 10(10):e0140531.
(14)Lili Lu,Lijuan Xu, Yuchuan Guo, Dayu Zhang, Tingting Qi, Lan Jin, Guofeng Gu, Li Xu, and Min Xiao*. Glycosylation of phenolic compounds by the site-mutated β-galactosidase fromLactobacillus bulgaricusL3.PLoS One.2015, 10(3):e0121445.
(15)Lili Zhang#,Lili Lu#Shuquan Fan#, Lan Jin, Guofeng Gu, Li Xu, and Min Xiao*. One-step synthesis of α-Gal epitope and globotriose derivatives by an engineered α-galactosidase,RSC Adv. 2015, 5, 22361-22364.
(16)Lili Lu, Feng Fu, Renfei Zhao, Lan Jin, Chunjuan He, Min Xiao*. A recombinant levansucrase fromBacillus licheniformis8-37-0-1 catalyzes versatile transfructosylation reactions.Process Biochem.2014, 49: 1503-1510.
(17)Lili Lu, Jian Wu, Deyong Song, Han Zhao, Yuchuan Guo, Guofeng Gu, Nan Jin, Min Xiao*. Purification of fructooligosaccharides by immobilized yeast cells and identification of a novel glycoside formed during the process.Bioresour Technol. 2013, 132: 365-369.
(18)Lili Lu, Shuze Xu, Renfei Zhao, Dayu Zhang, Zhengyi Li, Yumei Li, Min Xiao*. Synthesis of galactooligosaccharides by CBD fusion β-galactosidase immobilized on cellulose. Bioresource Technol. 2012, 116:327-333.
(19)Lili Lu, Shuze Xu, Lan Jin, Dayu Zhang, Yumei Li, Min Xiao*. Synthesis of galactosyl sucralose by β-galactosidase fromLactobacillus bulgaricusL3.Food Chem. 2012, 134(1): 269-275.
(20)Qian Liu#,Lili Lu#, Min Xiao*.Cell surface engineering of α-L-rhamnosidase for naringin hydrolysis. Bioresource Technol. 2012, 123: 144–149.
(21)Lili Lu#,Guofeng Gu#,Min Xiao*, Fengshan Wang. Separation andstructure analysis of trisaccharide isomers produced from lactose byLactobacillus bulgaricusL3β-galactosidase.Food Chem. 2010, 121(4):1283-1288.
(22)Lili Lu,Xiaodong Xu,Min Xiao*, Fengshan Wang.Synthesis of novel galactose-containing chemicals byβ-galactosidase fromEnterobacter cloacaeB5.Bioresource Technol. 2010, 101(17): 6868-6872.
(23)Yumei Li#,Lili Lu#,Hongmei Wang,Xiaodong Xu,Min Xiao*.Cell-surface engineering of a β-galactosidase for galactooligosaccharide synthesis.Appl Environ Microbiol. 2009, 75(18): 5938-5942.
(24)Lili Lu, Min Xiao*, Han Zhao, Xiaodong Xu, Fengshan Wang. Pharmacological chaperones: novel therapeutic strategies to rescue defective protein folding and trafficking.Prog Biochem Biophys.2008, 35(8): 875-885.
(25)Lili Lu, Min Xiao*, Xiaodong Xu, Zhengyi Li, Yumei Li.A novel β-galactosidase capable of glycosyl transfer fromEnterobacter agglomeransB1.Biochem Biophys Res Commun. 2007, 356 (1): 78-84.
(26)Lili Lu, Min xiao*, Zhengyi Li, Yumei Li, Fengshan wang. A novel transglycosylating β-galactosidase fromEnterobacter cloacaeB5.Process Biochem. 2009, 44 (2): 232-236.
(27)Lili Lu, Min Xiao*, Han Zhao, Peng Wang, Xinmin Qian. 1-Glycosyl fluorides in carbohydrate synthesis by chemoenzymatic methods.Chinese J Org chem.2006, 26 (12): 1631-1639.
(28)Lili Lu, Min Xiao*, Han Zhao, Peng Wang, Xinmin Qian. Glycosynthases: a novel efficient synthetic tool for oligosaccharides.Prog Biochem Biophys. 2006, 33 (4): 310-320.
代表性授权专利
(1)卢丽丽,齐亭亭,肖敏,徐莉.半乳糖型红景天苷及其衍生物的制备方法. ZL 2016 1 0979793.5
(2)卢丽丽,肖敏,郭玉川.间苯三酚糖苷衍生物及其制备方法.专利号: ZL 2013 1 0256401.9
(3)卢丽丽,肖敏,郭玉川.一种白藜芦醇半乳糖苷的制备方法.专利号: ZL 2013 1 0257340.8
(4)卢丽丽,肖敏,郭玉川.咖啡酸糖苷衍生物及其制备方法.专利号: ZL 2013 1 0257383.6
(5)卢丽丽,肖敏,郭玉川.一种邻苯二酚半乳糖苷的制备方法.专利号: ZL 2013 1 0255797.5
(6)肖敏,卢丽丽,郭玉川.一种能糖基化白藜芦醇的β-半乳糖苷酶.专利号: ZL2013 1 0256580.6
(7)肖敏,卢丽丽,章大雨,郭玉川.一种转苷底物特异性拓宽的β-半乳糖苷酶.专利号: ZL 2013 1 0257432.6
